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Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A12484) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Western blot analysis of extracts of 293T cells, using PIK3CA antibody (A12484) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human breast cancer using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded rat liver using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).
PIK3CA Rabbit pAb
Catalog No：AB-A12484
Manufacturer：ABclonal
Synonyms：PIK3CA;CLOVE;CWS5;MCAP;MCM;MCMTC;PI3K;PI3K-alpha
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PIK3CA Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. |
| Immunogen: | A synthetic peptide of human PIK3CA |
| Gene ID: | 5290 |
| Swiss prot: | P42336 |
| Molecular weight: | 124kDa |
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