Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 antibody (A0952) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
PIK3C3/VPS34 Rabbit pAb
Catalog No:AB-A0952
Manufacturer:ABclonal
Synonyms:PIK3C3;VPS34;Vps34;hVps34
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PIK3C3/VPS34 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PIK3C3/VPS34 (NP_002638.2). |
Gene ID: | 5289 |
Swiss prot: | Q8NEB9 |
Molecular weight: | 101kDa |
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