Western blot analysis of extracts of various cell lines, using PIAS3 antibody (A7060) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PIAS3 Rabbit pAb
Catalog No:AB-A7060
Manufacturer:ABclonal
Synonyms:PIAS3;ZMIZ5
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PIAS3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 452-628 of human PIAS3 (NP_006090.2). |
Gene ID: | 10401 |
Swiss prot: | Q9Y6X2 |
Molecular weight: | 68kDa |
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