Western blot analysis of extracts of various cell lines, using PIAS1 Rabbit mAb (A4744) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit mAb (A4744) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
PIAS1 Rabbit mAb
Catalog No:AB-A4744
Manufacturer:ABclonal
Synonyms:DDXBP1, GBP, GU/RH-II, ZMIZ3
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | PIAS1 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Immunogen: | A synthesized peptide derived from human PIAS1 |
Gene ID: | 8554 |
Swiss prot: | O75925 |
Molecular weight: | 76kDa |
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