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Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-ZAP70-Y319 antibody (AP0467).
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Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ZAP70-Y319 pAb (AP0467). Western blot was performed from the immunoprecipitate using Phospho-ZAP70-Y319 pAb (AP0467) at a dilition of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.
Phospho-ZAP70-Y319 Rabbit pAb
Catalog No：AB-AP0467
Manufacturer：ABclonal
Synonyms：ZAP70;ADMIO2;IMD48;SRK;STCD;STD;TZK;ZAP-70
Applications：WB,IHC,IF,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ZAP70-Y319 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around Y319 of human ZAP70 (NP_001070.2). |
| Gene ID: | 7535 |
| Swiss prot: | P43403 |
| Molecular weight: | 35kDa/55kDa/69kDa |
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