Western blot analysis of extracts of various cell lines, using phospho-YAP1-S397 antibody (AP0922) at 1:1000 dilution.NIH3T3cellsand C6cells were treated by Sorbitol (0.2M) at37℃ for 60 minutesafterserum-starvationovernight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 1s.
phospho-YAP1-S397 Rabbit pAb
Catalog No:AB-AP0922
Manufacturer:ABclonal
Synonyms:COB1;YAP;YAP2;YAP65;YKI;YAP1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | phospho-YAP1-S397 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S397 of human YAP1. |
Gene ID: | 10413 |
Swiss prot: | P46937 |
Molecular weight: | 36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa |
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