Western blot analysis of extracts of various cell lines, using Phospho-VEGF Receptor 2-Y1175 pAb (AP1095) at 1:1000 dilution.HUVEC cells were treated by VEGF (100 ng/mL) at 37℃ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-VEGF Receptor 2-Y1175 Rabbit pAb
Catalog No:AB-AP1095
Manufacturer:ABclonal
Synonyms:CD309;FLK1;VEGFR;VEGFR2;VEGF Receptor 2;KDR
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-VEGF Receptor 2-Y1175 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y1175 of human VEGF Receptor 2. |
Gene ID: | 3791 |
Swiss prot: | P35968 |
Molecular weight: | 75kDa/79kDa/151kDa |
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