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Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-VASP-S157 pAb (AP0763). Western blot was performed from the immunoprecipitate using Phospho-VASP-S157 pAb (AP0763) at a dilition of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.
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Western blot analysis of extracts of A431 cells, using Phospho-VASP-S157 antibody (AP0763) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat brain using Phospho-VASP-S157 antibody (AP0763) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using Phospho-VASP-S157 antibody (AP0763) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using Phospho-VASP-S157 antibody (AP0763) at dilution of 1:100 (40x lens).
Phospho-VASP-S157 Rabbit pAb
Catalog No：AB-AP0763
Manufacturer：ABclonal
Synonyms：VASP
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-VASP-S157 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
| Immunogen: | A synthetic phosphorylated peptide around S157 of human Phospho-VASP-S157 (NP_003361.1). |
| Gene ID: | 7408 |
| Swiss prot: | P50552 |
| Molecular weight: | 39kDa |
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