Western blot analysis of extracts of various cell lines, using Phospho-VANGL2-T78/S79/S82 antibody (AP1206) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by wnt(100ng/ml) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-VANGL2-T78/S79/S82 Rabbit mAb
Catalog No:AB-AP1206
Manufacturer:ABclonal
Synonyms:VANGL2;LPP1;LTAP;STB1;STBM;STBM1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€110,00 – €325,00
Product Name: | Phospho-VANGL2-T78/S79/S82 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a membrane protein involved in the regulation of planar cell polarity, especially in the stereociliary bundles of the cochlea. The encoded protein transmits directional signals to individual cells or groups of cells in epithelial sheets. This protein is also involved in the development of the neural plate. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T78/S79/S82 of human VANGL2 |
Gene ID: | 57216 |
Swiss prot: | Q9ULK5 |
Molecular weight: | 59kDa |
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