Western blot analysis of extracts of 293T cells, using Phospho-ULK1-S555 antibody (AP0760) at 1:1000 dilution.293T cells were treated by Oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-ULK1-S555 Rabbit pAb
Catalog No:AB-AP0760
Manufacturer:ABclonal
Synonyms:ULK1;ATG1;ATG1A;UNC51;Unc51.1;hATG1;ULk1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-ULK1-S555 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165). |
Immunogen: | A synthetic phosphorylated peptide around S555 of human Phospho-ULK1-S555 (NP_003556.1). |
Gene ID: | 8408 |
Swiss prot: | O75385 |
Molecular weight: | 112kDa |
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