Western blot analysis of extracts of C2C12 cells, using Phospho-TSC2-S1387 Rabbit pAb (AP1117) at 1:1000 dilution.C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-TSC2-S1387 Rabbit pAb
Catalog No:AB-AP1117
Manufacturer:ABclonal
Synonyms:TSC2; LAM; PPP1R160; TSC4; tuberin
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-TSC2-S1387 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S1387 of human Tuberin/TSC2. |
Gene ID: | 7249 |
Swiss prot: | P49815 |
Molecular weight: | 25kDa/187-200kDa |
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