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Western blot analysis of extracts of HeLa cells, using Phospho-TrkA-Y674/675 + TrkB-Y706/707 antibody (AP0549) at 1:500 dilution.HeLa cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-TrkA-Y674/675 + TrkB-Y706/707 Rabbit pAb
Catalog No：AB-AP0549
Manufacturer：ABclonal
Synonyms：NTRK1/NTRK2
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-TrkA-Y674/675 + TrkB-Y706/707 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y674 of human TrkA |
| Gene ID: | 4914/4915 |
| Swiss prot: | P04629/Q16620 |
| Molecular weight: | 35kDa/53-60kDa/77kDa/83-93kDa |
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