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Western blot analysis of extracts of THP-1 cells, using Phospho-TMEM173-S366 antibody (AP1223) at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37℃ for Serum-starvation overnight.THP-1 cells were differentiation with TPA (200 nM) for 15 minutes and transfection with poly(dA:dT) (5 ug/mL) for 3 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-TMEM173-S366 Rabbit pAb
Catalog No：AB-AP1223
Manufacturer：ABclonal
Synonyms：TMEM173;ERIS;MITA;MPYS;NET23;SAVI;STING;hMITA;hSTING
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-TMEM173-S366 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S366 of human TMEM173. |
| Gene ID: | 340061 |
| Swiss prot: | Q86WV6 |
| Molecular weight: |
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