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Western blot analysis of extracts of various cell lines, using Phospho-Tau-T181 antibody (U34897AP1234) at 1:500 dilution.U-251MG cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Mouse brain were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-Tau-T181 Rabbit pAb
Catalog No：AB-AP1234
Manufacturer：ABclonal
Synonyms：DDPAC;FTDP-17;MAPTL;MSTD;MTBT1;MTBT2;PPND;PPP1R103;TAU;MAPT;Tau
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-Tau-T181 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer’s disease, Pick’s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding T181 of Human Tau. |
| Gene ID: | 4137 |
| Swiss prot: | P10636-5 |
| Molecular weight: | 32-45kDa/78- 80kDa |
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