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Western blot analysis of extracts of K562 cells, using Phospho-STAT5-Y694 pAb (AP0887) at 1:1000 dilution.K-562 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat heart using Phospho-STAT5-Y694 antibody (AP0887) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using Phospho-STAT5-Y694 antibody (AP0887) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Phospho-STAT5-Y694 antibody (AP0887) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of A-431 cells, using Phospho-STAT5-Y694 pAb (AP0887) at 1:1000 dilution or STAT5B antibody (A12356).A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-STAT5-Y694 Rabbit pAb
Catalog No：AB-AP0887
Manufacturer：ABclonal
Synonyms：MGF;STAT5;stat5a/stat5b
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-STAT5-Y694 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y694 of human STAT5. |
| Gene ID: | 6776/6777 |
| Swiss prot: | P42229/P51692 |
| Molecular weight: | 90kDa |
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