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Immunofluorescence analysis of rat brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded Rat brain using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human liver cancer using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Mouse liver using Phospho-STAT3-Y705 antibody (AP0070) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-Y705 antibody (AP0070) at 1:500 dilution.HeLa cells were treated by IFN-ɑ (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-STAT3-Y705 Rabbit pAb
Catalog No：AB-AP0070
Manufacturer：ABclonal
Synonyms：ADMIO;ADMIO1;APRF;HIES;STAT3;Stat3
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-STAT3-Y705 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Immunogen: | A synthetic phosphorylated peptide around Y705 of human Phospho-STAT3-Y705 (NP_644805.1). |
| Gene ID: | 6774 |
| Swiss prot: | P40763 |
| Molecular weight: | 83kDa/87kDa/88kDa |
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