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Western blot analysis of extracts of various cell lines, using Phospho-STAT2-Y690 pAb (AP0284) at 1:1000 dilution.NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-STAT2-Y690 Rabbit pAb
Catalog No：AB-AP0284
Manufacturer：ABclonal
Synonyms：STAT2;IMD44;ISGF-3;P113;STAT113
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-STAT2-Y690 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around Y690 of human STAT2 (NP_005410.1). |
| Gene ID: | 6773 |
| Swiss prot: | P52630 |
| Molecular weight: | 97kDa |
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