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Western blot analysis of extracts of various cell lines, using Phospho-STAT1-S727 antibody (AP0109) at 1:2000 dilution. 293T cells and C6 cells were treated by UV at room temperature for 15-30 minutes..|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded human esophageal using Phospho-STAT1-S727 Rabbit pAb (AP0109) at dilution of 1:100 (40x lens).
Phospho-STAT1-S727 Rabbit pAb
Catalog No：AB-AP0109
Manufacturer：ABclonal
Synonyms：CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;STAT91;STAT1 alpha;STAT1
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-STAT1-S727 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Immunogen: | A synthetic phosphorylated peptide around S727 of human STAT1 (NP_009330.1). |
| Gene ID: | 6772 |
| Swiss prot: | P42224 |
| Molecular weight: | 83kDa/87kDa |
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