Western blot analysis of extracts of various cell lines, using Phospho-Src-Y529 pAb (AP0185) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour or treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-Src-Y529 Rabbit pAb
Catalog No:AB-AP0185
Manufacturer:ABclonal
Synonyms:ASV;SRC1;THC6;c-SRC;p60-Src;SRC
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Src-Y529 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
Immunogen: | A synthetic phosphorylated peptide around Y529 of human Phospho-Src-Y529 (NP_005408.1). |
Gene ID: | 6714 |
Swiss prot: | P12931 |
Molecular weight: | 59kDa/60kDa |
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