Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (AP1027) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-Src-Y419 Rabbit mAb
Catalog No:AB-AP1027
Manufacturer:ABclonal
Synonyms:ASV, SRC1, THC6, c-SRC, p60-Src
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-Src-Y419 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y419 of human Phospho-Src-Y419 |
Gene ID: | 6714 |
Swiss prot: | P12931 |
Molecular weight: | 60kDa |
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