Western blot analysis of extracts of Jurkat cells, using Phospho-SMO-S611/615 antibody (AP0939) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Jurkat cells were treated by Anisomycin (25 ug/mL) at 37℃ for 20 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-SMO-S611/615 Rabbit pAb
Catalog No:AB-AP0939
Manufacturer:ABclonal
Synonyms:Gx;CRJS;SMOH;FZD11;SMO
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-SMO-S611/615 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. |
Immunogen: | A synthetic phosphorylated peptide around S611/615 of human SMO. |
Gene ID: | 6608 |
Swiss prot: | Q99835 |
Molecular weight: | 86kDa |
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