Western blot analysis of extracts of 293T cells, using Phospho-SMC1-S957 Rabbit mAb (AP1145) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SMC1-S957 Rabbit mAb (AP1145) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-SMC1-S957 Rabbit mAb
Catalog No:AB-AP1145
Manufacturer:ABclonal
Synonyms:CDLS2, DXS423E, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-SMC1-S957 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S957 of human SMC1 |
Gene ID: | 8243 |
Swiss prot: | Q14683 |
Molecular weight: | 160kDa |
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