Western blot analysis of extracts of HeLa cells, using Phospho-Smad5-S463/S465 antibody (AP1023) at 1:1000 dilution or Smad5 antibody (A1947).Hela cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-Smad5-S463/S465 Rabbit mAb
Catalog No:AB-AP1023
Manufacturer:ABclonal
Synonyms:DWFC, JV5-1, MADH5
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-Smad5-S463/S465 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S463/S465 of human Smad5 |
Gene ID: | 4090 |
Swiss prot: | Q99717 |
Molecular weight: | 52kDa |
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