Western blot analysis of extracts of L929 cells, using Phospho-RIP3-T231/S232 antibody (AP1260) at 1:500 dilution.L929 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-RIP3-T231/S232 Rabbit pAb
Catalog No:AB-AP1260
Manufacturer:ABclonal
Synonyms:R; Rip3; AW107945; 2610528K09Rik
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€150,00 – €410,00
Product Name: | Phospho-RIP3-T231/S232 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T231/S232 of Mouse Ripk3. |
Gene ID: | 56532 |
Swiss prot: | Q9QZL0 |
Molecular weight: | 53kDa |
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