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Western blot analysis of extracts of HeLa cells, using Phospho-RB-S811 antibody (AP0089) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
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Immunofluorescence analysis of U2OS cells using Phospho-Rb-S811 antibody (AP0089). Blue: DAPI for nuclear staining.
Phospho-RB-S811 Rabbit pAb
Catalog No：AB-AP0089
Manufacturer：ABclonal
Synonyms：OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-RB-S811 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
| Immunogen: | A synthetic phosphorylated peptide around S811 of human RB (NP_000312.2). |
| Gene ID: | 5925 |
| Swiss prot: | P06400 |
| Molecular weight: | 106kDa |
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