Western blot analysis of extracts of HT-29 cells, using Phospho-RB-S807/811 antibody (AP0484) at 1:1000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-RB-S807/811 Rabbit pAb
Catalog No:AB-AP0484
Manufacturer:ABclonal
Synonyms:OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-RB-S807/811 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
Immunogen: | A synthetic phosphorylated peptide around S807 & S811 of human RB (NP_000312.2). |
Gene ID: | 5925 |
Swiss prot: | P06400 |
Molecular weight: | 106kDa |
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