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Western blot analysis of extracts of HeLa cells, using Phospho-Raf1-S259 antibody (AP0497) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-Raf1-S259 Rabbit pAb
Catalog No：AB-AP0497
Manufacturer：ABclonal
Synonyms：CMD1NN;CRAF;NS5;Raf-1;c-Raf;RAF1
Applications：WB,IP
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Raf1-S259 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. |
| Immunogen: | A synthetic phosphorylated peptide around S259 of human Phospho-Raf1-S259 (NP_002871.1). |
| Gene ID: | 5894 |
| Swiss prot: | P04049 |
| Molecular weight: | 73kDa/75kDa |
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