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Western blot analysis of extracts of A-431 cells, using Phospho-Rac1/2/3-S71 antibody (AP0086) at 1:1000 dilution.A-431 cells were treated by EGF (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-Rac1/2/3-S71 Rabbit pAb
Catalog No：AB-AP0086
Manufacturer：ABclonal
Synonyms：RAC1;MIG5;Rac-1;TC-25;p21-Rac1
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Rac1/2/3-S71 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S71 of human RAC1 (NP_008839.2). |
| Gene ID: | 5879 |
| Swiss prot: | P63000 |
| Molecular weight: | 21kDa/23kDa |
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