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Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-T853 antibody ( AP0916) at 1:1000 dilution.Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-PPP1R12A-T853 Rabbit pAb
Catalog No：AB-AP0916
Manufacturer：ABclonal
Synonyms：PPP1R12A;M130;MBS;MYPT1;MYPT1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PPP1R12A-T853 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around T853 of human PPP1R12A. |
| Gene ID: | 4659 |
| Swiss prot: | O14974 |
| Molecular weight: | 105kDa/109kDa/111kDa/115kDa |
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