.jpg)
Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-S507 antibody (AP0769) at 1:2000 dilution or PPP1R12A antibody (A0587). HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PPP1R12A-S507 Rabbit pAb
Catalog No：AB-AP0769
Manufacturer：ABclonal
Synonyms：PPP1R12A;M130;MBS;MYPT1;MYPT1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PPP1R12A-S507 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1). |
| Gene ID: | 4659 |
| Swiss prot: | O14974 |
| Molecular weight: | 105kDa/109kDa/111kDa/115kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No：AB-AP0023
Manufacturer：ABclonal
Synonyms：EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0022
Manufacturer：ABclonal
Synonyms：ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0012
Manufacturer：ABclonal
Synonyms：BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0011
Manufacturer：ABclonal
Synonyms：BAD;BBC2;BCL2L8;Bad
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0009
Manufacturer：ABclonal
Synonyms：BAD;BBC2;BCL2L8;Bad
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0004
Manufacturer：ABclonal
Synonyms：AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00