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Western blot analysis of extracts of various cell lines, using Phospho-POLR2A-S5 Rabbit mAb (AP0997) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours or treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-POLR2A-S5 Rabbit mAb
Catalog No：AB-AP0997
Manufacturer：ABclonal
Synonyms：POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-POLR2A-S5 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A |
| Gene ID: | 5430 |
| Swiss prot: | P24928 |
| Molecular weight: | 270kDa |
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