Western blot analysis of extracts of HT-29 cells, using Phospho-PLK1-T210 (AP1025) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37℃ for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-PLK1-T210 Rabbit mAb
Catalog No:AB-AP1025
Manufacturer:ABclonal
Synonyms:PLK, STPK13
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-PLK1-T210 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T210 of human PLK1 |
Gene ID: | 5347 |
Swiss prot: | P53350 |
Molecular weight: | 68kDa |
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