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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PLA2G4A-S505 antibody (AP0968) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-PLA2G4A-S505 Rabbit pAb
Catalog No：AB-AP0968
Manufacturer：ABclonal
Synonyms：PLA2G4A;PLA2G4;cPLA2;cPLA2-alpha
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €425,00
| Product Name: | Phospho-PLA2G4A-S505 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic phosphorylated peptide around of human Phospho-PLA2G4A-S505. |
| Gene ID: | 5321 |
| Swiss prot: | P47712 |
| Molecular weight: | 85kDa |
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