.jpg)
Western blot analysis of extracts of various cell lines, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (A4869) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
.jpg)
Western blot analysis of extracts of various cell lines, using Phospho-PKR/EIF2AK2-T446 Rabbit mAb (A4869) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-PKR/EIF2AK2-T446 Rabbit mAb
Catalog No：AB-AP1134
Manufacturer：ABclonal
Synonyms：EIF2AK1, PKR, PPP1R83, PRKR
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €525,00
| Product Name: | Phospho-PKR/EIF2AK2-T446 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T446 of human PKR/EIF2AK2 |
| Gene ID: | 5610 |
| Swiss prot: | P19525 |
| Molecular weight: | 62kDa |
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