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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKM2-Y105 pAb (AP0924) at 1:1000 dilution or PKM antibody (A13905).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PKM2-Y105 Rabbit pAb
Catalog No：AB-AP0924
Manufacturer：ABclonal
Synonyms：CTHBP;HEL-S-30;OIP3;PK3;PKM2;TCB;THBP1;PKM
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PKM2-Y105 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y105 of human PKM. |
| Gene ID: | 5315 |
| Swiss prot: | P14618 |
| Molecular weight: | 58kDa |
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