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Western blot analysis of extracts of various cell lines, using Phospho-PKC zeta-T560 Rabbit mAb (AP1149) at 1:1000 dilution.Both 293T cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Phospho-PKC zeta-T560 Rabbit mAb
Catalog No：AB-AP1149
Manufacturer：ABclonal
Synonyms：PKC-ZETA, PKC2
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-PKC zeta-T560 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T560 of human PKC zeta |
| Gene ID: | 5590 |
| Swiss prot: | Q05513 |
| Molecular weight: | 68kDa |
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