Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PKC mu-S744/748 pAb (AP0534) at 1:1000 dilution or PRKD1 antibody (A16185).NIH/3T3 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-PKC mu-S744/748 Rabbit pAb
Catalog No:AB-AP0534
Manufacturer:ABclonal
Synonyms:CHDED;PKC-MU;PKCM;PKD;PRKCM;PRKD1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-PKC mu-S744/748 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S744/748 of human PKC mu |
Gene ID: | 5587 |
Swiss prot: | Q15139 |
Molecular weight: | 101kDa |
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