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Western blot analysis of extracts of 293 cells, using Phospho-PKC alpha-S226 antibody (AP0559) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-PKC alpha-S226 Rabbit pAb
Catalog No：AB-AP0559
Manufacturer：ABclonal
Synonyms：PRKCA;AAG6;PKC-alpha;PKCA;PRKACA;PKC alpha
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PKC alpha-S226 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. |
| Immunogen: | A synthetic phosphorylated peptide around S226 of human PKC alpha (NP_002728.2). |
| Gene ID: | 5578 |
| Swiss prot: | P17252 |
| Molecular weight: | 76kDa |
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