Western blot analysis of extracts of 293T cells, using Phospho-PKA RIIɑ (PRKAR2A)-S99 Rabbit mAb (AP1034) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of C6 cells, using Phospho-PKA RIIɑ (PRKAR2A)-S99 Rabbit mAb (AP1034) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-PKA RIIα (PRKAR2A)-S99 Rabbit mAb
Catalog No:AB-AP1034
Manufacturer:ABclonal
Synonyms:PKR2, PRKAR2
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-PKA RII伪 (PRKAR2A)-S99 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S99 of human PRKAR2A/PKR2 |
Gene ID: | 5576 |
Swiss prot: | P13861 |
Molecular weight: | 51kDa |
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