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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-PKA C-alpha (PRKACA)-S339 pAb (AP0558). Western blot was performed from the immunoprecipitate using Phospho-PKA C-alpha (PRKACA)-S339 pAb (AP0558) at a dilition of 1:1000. HeLa cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
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Western blot analysis of extracts of HeLa and 293 cells, using Phospho-PKA C-alpha (PRKACA)-S339 antibody (AP0558) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-PKA C-alpha (PRKACA)-S339 Rabbit pAb
Catalog No：AB-AP0558
Manufacturer：ABclonal
Synonyms：PRKACA;PKACA;PPNAD4
Applications：WB,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PKA C-alpha (PRKACA)-S339 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing’s syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. |
| Immunogen: | A synthetic phosphorylated peptide around S339 of human PKA C-alpha (PRKACA) (NP_002721.1). |
| Gene ID: | 5566 |
| Swiss prot: | P17612 |
| Molecular weight: | 39kDa/40kDa |
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