Western blot analysis of extracts of 293T cells, using Phospho-PI3KP85ɑ/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of 293T cells, using Phospho-PI3KP85ɑ/γ/β-Y467/Y199/Y464 antibody (AP0854) at 1:1000 dilution.293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PI3KP85α/γ/β-Y467/Y199/Y464 Rabbit pAb
Catalog No:AB-AP0854
Manufacturer:ABclonal
Synonyms:PIK3R1/PIK3R2/PIK3R3
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-PI3KP85伪/纬/尾-Y467/Y199/Y464 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
Immunogen: | A synthetic phosphorylated peptide around Y467/Y199/Y464 of human p85-alpha (NP_852664.1). |
Gene ID: | 5295/5296/8503 |
Swiss prot: | P27986/O00459/Q92569 |
Molecular weight: | 42-54kDa/81-84kDa |
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