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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y771 pAb (AP1062) at 1:1000 dilution or Phospho-PDGFRB-Y771 antibody (A2180).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-PDGFRB-Y771 Rabbit pAb
Catalog No：AB-AP1062
Manufacturer：ABclonal
Synonyms：CD140B;IBGC4;IMF1;JTK12;KOGS;PDGFR;PDGFR-1;PDGFR1;PENTT;PDGF Receptor beta;PDGFRB;PDGFR beta
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PDGFRB-Y771 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding Y771 of human Phospho-PDGFRB-Y771. |
| Gene ID: | 5159 |
| Swiss prot: | P09619 |
| Molecular weight: | 124kDa |
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