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Western blot analysis of extracts of HeLa cells, using Phospho-Paxillin-Y118 pAb (AP1057) at 1:1000 dilution or Paxillin antibody (A18181).HeLa cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Paxillin-Y118 Rabbit pAb
Catalog No：AB-AP1057
Manufacturer：ABclonal
Synonyms：Paxillin;PXN;paxillin
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Paxillin-Y118 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding Y118 of human Paxillin. |
| Gene ID: | 5829 |
| Swiss prot: | P49023 |
| Molecular weight: | 64kDa |
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