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Western blot analysis of extracts of various cell lines, using Phospho-Paxillin-Y118 Rabbit mAb (AP1156) at 1:1000 dilution.A-431 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Paxillin-Y118 Rabbit mAb
Catalog No：AB-AP1156
Manufacturer：ABclonal
Synonyms：PXN; paxillin
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Paxillin-Y118 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y118 of human Phospho-Paxillin-Y118 |
| Gene ID: | 5829 |
| Swiss prot: | P49023 |
| Molecular weight: | 68kDa |
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