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Western blot analysis of extracts of various cell lines, using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb
Catalog No：AB-AP1049
Manufacturer：ABclonal
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S474 of human PAK4 |
| Gene ID: | 10298/57144/56924 |
| Swiss prot: | O96013(PAK4-Antigen),Q9P286(PAK5),Q9NQU5(PAK6) |
| Molecular weight: | 64, 80, 75 kDa |
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