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Western blot analysis of extracts of HeLa cells, using Phospho-PAK2-S20 antibody (AP0803) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PAK2-S20 pAb (AP0803) at 1:2000 dilution or PAK2 antibody (A7333).NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-PAK2-S20 Rabbit pAb
Catalog No：AB-AP0803
Manufacturer：ABclonal
Synonyms：PAK2;PAK65;PAKgamma
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-PAK2-S20 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. |
| Immunogen: | A synthetic phosphorylated peptide around S20 of human PAK2 (NP_002568.2). |
| Gene ID: | 5062 |
| Swiss prot: | Q13177 |
| Molecular weight: | 58kDa |
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