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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T421/S424 pAb (AP1106) at 1:1000 dilution or P70 S6K antibody (A16968).NIH/3T3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb
Catalog No：AB-AP1106
Manufacturer：ABclonal
Synonyms：PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T421/S424 of human RPS6KB1. |
| Gene ID: | 6198 |
| Swiss prot: | P23443 |
| Molecular weight: | 51kDa/52kDa/56kDa/59kDa |
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