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Western blot analysis of extracts of 293T cells, using Phospho-p70 S6 Kinase 1-T421/S424 antibody (AP0540) at 1:2000 dilution. 293T cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb
Catalog No：AB-AP0540
Manufacturer：ABclonal
Synonyms：PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T421/S424 of human P70S6K1 |
| Gene ID: | 6198 |
| Swiss prot: | P23443 |
| Molecular weight: | 51kDa/52kDa/56kDa/59kDa |
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