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Western blot analysis of extracts of 293T cells, using Phospho-p53-T55 Rabbit mAb (AP0986) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-p53-T55 Rabbit mAb
Catalog No：AB-AP0986
Manufacturer：ABclonal
Synonyms：BCC7, LFS1, P53, TRP53
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-p53-T55 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T55 of human p53 |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 53kDa |
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