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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb (AP0985) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-p53-S9 Rabbit mAb
Catalog No：AB-AP0985
Manufacturer：ABclonal
Synonyms：BCC7, LFS1, P53, TRP53
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €525,00
| Product Name: | Phospho-p53-S9 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S9 of human p53 |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 53kDa |
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